216623b
Laborpraktikum
WiSe 22/23: Funktionelle Genomik mit CRISPR
Edda Schulz
Kommentar
Inhalt/Content:
The focus of the course might be shifted towards computational tools and data analysis, if the conditions do not allow extensive lab work at that time.
- Computational analysis of next generation sequencing data (mapping, quality control, quantification)
- Computational analysis of CRISPR screening data
- Data analysis in R
- sgRNA design
- CRISPR screen design
- RNA extraction
- cDNA generation through reverse transcription
- Quantitative PCR
- Flow cytometry
- embryonic stem cell culture
- lentiviral transduction
Dr. E. Schulz: edda.schulz@molgen.mpg.de Schließen
The focus of the course might be shifted towards computational tools and data analysis, if the conditions do not allow extensive lab work at that time.
- Computational analysis of next generation sequencing data (mapping, quality control, quantification)
- Computational analysis of CRISPR screening data
- Data analysis in R
- sgRNA design
- CRISPR screen design
- RNA extraction
- cDNA generation through reverse transcription
- Quantitative PCR
- Flow cytometry
- embryonic stem cell culture
- lentiviral transduction
Dr. E. Schulz: edda.schulz@molgen.mpg.de Schließen
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