UN Sustainable Development Goals (SDGs): 3, 6, 14, 15

Content:

Lecture:
Bacterial adaptation mechanisms to stress, starvation and other growth-limiting conditions, microbial biofilms, microbial redox physiology, signal transduction and regulation of gene expression, mechanisms of microbial pathogenicity, protein quality control, functional genomic analysis of bacteria (genome sequencing, transcriptomics, microbial proteomics). 



Practical course:
Regulation of gene expression after the influence of stress (reporter genes lacZ fusions, ß-galactosidase determination; Northern blot analyses) Molecular biology/genetic engineering (PCR, cloning, blue/white screening in E. coli, mutant construction in B. subtilis, overexpression and Affinity purification of recombinant proteins in E. coli, measurement of enzyme activities of redox-sensitive recombinant proteins (GapDH), measurement of roGFP2 redox sensors Bacterial genomes, transcriptomes, proteomes and bioinformatic analysis of proteome data (Western blot analyses, gel-free and gel-based proteome analyses, bioinformatics)


Seminar:
Theoretical and methodological introduction to the internship experiments, presentation of results from the internship experiments and their critical discussion, taking into account generally accepted theories and findings as well as the methods and techniques used.




Qualification goals:

The module provides a wide range of in-depth knowledge of physiology, genetics, molecular biology and functional genomic research of prokaryotic microorganisms (microbial proteomics and transcription analyses). After completing the module, the students are able to identify, formulate and discuss subject-specific questions, design experimental strategies to solve them and plan and carry out corresponding experiments independently.

The exam on the content of V, S, Ü+P will take place in the last week on February 7th, 2025.

1. FUCHS, G. (Hrsg.) Allgemeine Mikrobiologie, begründet von Hans G. SCHLEGEL, 9., vollständig überarbeitete und erweiterte Auflage 2014, Georg Thieme Verlag, Stuttgart, New York.
2. MUNK, K. (Hrsg.) Taschenlehrbuch Biologie: Mikrobiologie. 1. Auflage, Georg Thieme Verlag, 2008, Stuttgart.
3. MADIGAN, M. T., J. M. MARTINKO, D. A. STAHL and D. P. CLARK. Brock Biology of Microorganisms, 13th edition, Pearson Education, Inc., 2012, San Francisco.
4. MADIGAN, M. T., J. M. MARTINKO, D. A. STAHL und D. P. CLARK. Brock Mikrobiologie, 13., aktualisierte Auflage, Pearson Higher Education, 2013, München.
5. Lottspeich, F. und J.W.Engel (Hrsg) Bioanalytik. 2.Auflage, Spektrum, Akademischer Verlag, Elsevier GmbH, München, 2006
6. Special literature reviews to the topics are made available