Inhalt/Content:
The focus of the course might be shifted towards computational tools and data analysis, if the conditions do not allow extensive lab work at that time.

- Computational analysis of next generation sequencing data (mapping, quality control, quantification)
- Computational analysis of CRISPR screening data
- Data analysis in R
- sgRNA design
- CRISPR screen design
- RNA extraction
- cDNA generation through reverse transcription
- Quantitative PCR
- Flow cytometry
- embryonic stem cell culture
- lentiviral transduction


Dr. E. Schulz: edda.schulz@molgen.mpg.de close