Cancelled
216451a
Seminar
SoSe 23: Membrane Protein Expression in Cell-Free Systems
Stefan Kubick
Information for students
Additional information / Pre-requisites
Attention!: Participation in the lecture and seminar 216501 a, b in an earlier semester are prerequesites for the enrollment in this course!
Comments
Content
- Cell-free Protein Synthesis in pro- and eukaryotic Systems
- Cell-free Protein Synthesis in pharmaceutical Protein Research and Production
- Cell-free coupled and linked transcription/translation Systems
- Batch-formatted Reactions and Dialysis (CECF)-Systems
- High-Throughput Membrane Protein Expression using Cell-free Systems
- Cell-free Synthesis of toxic Proteins, labelled Proteins and Protein Conjugates
- Cell-free Synthesis of “difficult-to-express” Proteins
- Cell-free Antibody-Production
- Chaperones, Nanodiscs and Detergents in Cell-free Systems
- Qualitative and quantitative Analysis of cell-free produced Membrane Proteins
- (Autoradiography and hot TCA Precipitation)
- posttranslational Modification Pathways in Cell-free Systems
- Generation of DNA-Templates by Expression-PCR
- Co-translational Translocation in eukaryotic Cell-free Systems
- Effects of Signal-Sequences in eukaryotic Cell-free Systems
- Functional Analysis of Cell-free produced Ionchannels and GPCRs
- Preparation of Giant Unilamelar Vesicles (GUVs)
- Single-Molecule-Analysis of membrane-embedded Proteins
- In vitro Synthesis of Glycoproteins
- Site-specific Incorporation of non-canonical Amino Acids
- Orthogonal Systems
Dr. S. Kubick: stefan.kubick@fu-berlin.de