23410a
Lecture
SoSe 24: V Mechanismen der mikrobiellen Stressanpassung
Haike Antelmann, Eberhard Klauck
Information for students
Additional information / Pre-requisites
The exam will take place on May 17, 2024 at 10 a.m.
Comments
Lecture: Deepening on the lecture Mol. Microbiology and microbial physiology and discussion of current literature on topics in molecular microbiology.
Topics: Bacterial adaptation mechanisms to stress, starvation and other growth-limiting conditions, microbial biofilms, microbial redox physiology, signal transduction and regulation of gene expression, mechanisms of microbial pathogenicity, protein quality control, functional genomic analysis of bacteria (genome sequencing, transcriptomics, microbial proteomics).
Practical course: Regulation of gene expression after the stress exposure (reporter genes lacZ fusions, ß-galactosidase determination; Northern blot analyses) Molecular biology/genetic engineering (PCR, cloning, blue/white screening in E. coli, mutant construction in B. subtilis, overexpression and Affinity purification of recombinant proteins in E. coli, measurement of enzyme activities of redox-sensitive recombinant proteins (GapDH), measurement of roGFP2 redox sensors Bacterial genomes, transcriptomes, proteomes and bioinformatic analysis of proteome data (Western blot analyses, gel-free and gel-based proteome analyses, bioinformatics).
Seminar: Theoretical and methodological introduction to the experiments of the practical course. Presentation of results from the experiments and their critical discussion, taking into account generally accepted theories and findings as well as the applied methods and techniques. close
Topics: Bacterial adaptation mechanisms to stress, starvation and other growth-limiting conditions, microbial biofilms, microbial redox physiology, signal transduction and regulation of gene expression, mechanisms of microbial pathogenicity, protein quality control, functional genomic analysis of bacteria (genome sequencing, transcriptomics, microbial proteomics).
Practical course: Regulation of gene expression after the stress exposure (reporter genes lacZ fusions, ß-galactosidase determination; Northern blot analyses) Molecular biology/genetic engineering (PCR, cloning, blue/white screening in E. coli, mutant construction in B. subtilis, overexpression and Affinity purification of recombinant proteins in E. coli, measurement of enzyme activities of redox-sensitive recombinant proteins (GapDH), measurement of roGFP2 redox sensors Bacterial genomes, transcriptomes, proteomes and bioinformatic analysis of proteome data (Western blot analyses, gel-free and gel-based proteome analyses, bioinformatics).
Seminar: Theoretical and methodological introduction to the experiments of the practical course. Presentation of results from the experiments and their critical discussion, taking into account generally accepted theories and findings as well as the applied methods and techniques. close
Suggested reading
1. FUCHS, G. (Hrsg.) Allgemeine Mikrobiologie, begründet von Hans G. SCHLEGEL, 9., vollständig überarbeitete und erweiterte Auflage 2014, Georg Thieme Verlag, Stuttgart, New York.