This seminar is one part of the methods course "Quantitative Fluorescence Microscopy" and takes place in parallel to the practical course. The seminar can be taken as theoretical module for the area structural biochemistry. close

Learning aims: The students will develop an overview of the capabilities of modern light microscopy and a basic understanding of the role of sample preparation and imaging in the formation of quantitative digital data. The students will get insight into the basics of computational image analysis and how data can be extracted from digital image data. The students will learn how to analyze digital images and how to recognize and avoid mistakes in image analysis. Contents: - Fundamentals of fluorescence microscopy - Basics of digital imaging - Fundamental image analysis - Special methods in microscopy - Single molecule microscopy - superresolution microscopy - Fluorescence resonant energy transfer - Machine learning in image analysis Prof. Dr. H. Ewers: helge.ewers@fu-berlin.de Dr. Bas van Bommel: bas.van.bommel@fu-berlin.de close