216621b
Laboratory Course
SoSe 24: Analyzing musculoskeletal development in vivo
Sigmar Stricker
Information for students
Recommendation: It is recommended to complete module 216701 a, b "Introduction to developmental biology" before taking the methods module.
Attendance of the seminar is obligatory for the attendance of the practical course
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Attendance of the seminar is obligatory for the attendance of the practical course
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Comments
Content:
Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close
- Dissection of laboratory mice
- Tissue embedding, cryoconservation
- Cryosectioning of tissue
- Histological methods; Hematoxylin/Eosin staining, Oil Red O staining of tissue sections
- Alizarin red / Alcian blue staining of skeletal preparations
- PCR-Genotyping of genetically modified laboratory mice
- Immunohistochemistry
- mRNA in-situ hybridization
- imaging of whole-embryo specimen
- imaging of histological sections
- imaging of fluorescently labelled tissue sections
Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close