216405b
Laboratory Course
SoSe 24: Mechanisms of Alternative Splicing Regulation
Florian Heyd, Marco Preußner
Information for students
Participation in the seminar is a prerequisite for participation in the laboratory course.
Comments
Content:
Prof. Dr. F. Heyd: florian.heyd@fu-berlin.de Dr. Marco Preußner: mpreussner@zedat.fu-berlin.de close
- Safe handling of radioisotopes
- Chemical synthesis of oligonucleotides
- Purification of oligonucleotides via HPLC
- Purification of oligonucleotides via PAGE
- Radioactive Labeling of oligonucleotides
- Single-turnover kinetics on hammerhead ribozymes
- Purification of target proteins
- Synthesis of a combinatorial RNA library
- Enrichment of oligoribonucleotides binding to a target
- cDNA synthesis via reverse transcription
- PCR amplification
Prof. Dr. F. Heyd: florian.heyd@fu-berlin.de Dr. Marco Preußner: mpreussner@zedat.fu-berlin.de close