The block course is aimed at students with an interest in research-relevant questions of structure elucidation on synthetic supramolecular architectures as well as biological macromolecules and introduces in particular transmission electron microscopy (TEM) in cryo mode. In the first part, questions of preparation, in particular cryofixation, basics of equipment design and function, imaging and evaluation, principles of digital image processing, and handling of transmission electron microscopes are covered theoretically and practically. The second part covers theoretical and practical aspects of modern structure elucidation by means of single particle analysis and enables the students to understand the formation of a high-resolution 3D reconstruction of a protein structure.

Dr. Kai Ludwig: kai.ludwig@fzem.fu-berlin.de
Dr. Tarek Hilal: tarek.hilal@fzem.fu-berlin.de close