216201a
Seminar
Biomolecular X-Ray Crystallography
Markus Wahl; Berhard Loll
Information for students
Part of the method module "Grundlagen der Strukturbiochemie". It is possible to attend this module-part as a theoretical module in the field of structural biochemistry.
Comments
Method module: Fundamentals of Structural Biochemistry
University/Department/Institute: Freie Universität Berlin/Department of Biology, Chemistry, Pharmacy/Institute of Chemistry and Biochemistry
Person responsible for the module: Lecturers of the module
Admission requirements: none
Qualification goals: Students learn methods for the preparation of biological macromolecules and for structural analysis. They acquire the theoretical basics of macromolecular structural analysis methods. Students are able to assess macromolecular properties that play a role in structural analysis. They will be able to critically evaluate manuscripts in which macromolecular structures and structural analyses are described and assess the quality of macromolecular structures. Contents: Preparation of a sample of a biological macromolecule for structural analysis; bioinformatic, biochemical or biophysical characterization of a biological macromolecule; performance of one or more procedures for structural analysis; structural description and graphical representation of structures; presentation of structural biology experiments and results.
Content of the seminar:
Protein production and characterization
Protein crystallization and protein crystals
Crystallographic symmetry
X-ray diffraction theory
The phase problem and phasing strategies
Electron density, model building, refinement, validation
close
University/Department/Institute: Freie Universität Berlin/Department of Biology, Chemistry, Pharmacy/Institute of Chemistry and Biochemistry
Person responsible for the module: Lecturers of the module
Admission requirements: none
Qualification goals: Students learn methods for the preparation of biological macromolecules and for structural analysis. They acquire the theoretical basics of macromolecular structural analysis methods. Students are able to assess macromolecular properties that play a role in structural analysis. They will be able to critically evaluate manuscripts in which macromolecular structures and structural analyses are described and assess the quality of macromolecular structures. Contents: Preparation of a sample of a biological macromolecule for structural analysis; bioinformatic, biochemical or biophysical characterization of a biological macromolecule; performance of one or more procedures for structural analysis; structural description and graphical representation of structures; presentation of structural biology experiments and results.
Content of the seminar:
Protein production and characterization
Protein crystallization and protein crystals
Crystallographic symmetry
X-ray diffraction theory
The phase problem and phasing strategies
Electron density, model building, refinement, validation
close