216201c
Laboratory Course
Biomolecular X-Ray Crystallography
Oliver Daumke, Bernhard Loll, Markus Wahl, Gert Weber, Manfred Weiss
Information for students
Teilmodul des Methodenkurses "Grundlagen der Strukturbiochemie"
Additional information / Pre-requisites
Additional informations:
Part 1: Wahl, Loll
Schedule:.28.10 - 08.11.2024
Location: Takustr. 6, 3. OG, Wahl group
Part 2: Weiss Important note: Pregnant and breastfeeding women are prohibited from working on the storage ring (Part 2) due to radiation protection regulations.
Schedule: 11.11. - 15.11.2024, Meeting point at 10:00 am at the gatekeeper
Location: c/o Soft Matter and Functional Materials, Elektronenspeicherring BESSY II, Albert-Einstein-Str. 15, 12489 Berlin, Adlershof
Part 3: Daumke
Schedule: 18.11. - 21.11..2024
Location: MDC für Molekulare Medizin, Robert-Rössle-Str. 10. 13125 Berlin (Buch), Seminar: MDC.C (Haus 83). Dendrit 2; Praktikum: Haus 31.2, Raum 0248 (AG Heinemann) (s.Vorlesungsverzeichnis)
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Part 1: Wahl, Loll
Schedule:.28.10 - 08.11.2024
Location: Takustr. 6, 3. OG, Wahl group
Part 2: Weiss Important note: Pregnant and breastfeeding women are prohibited from working on the storage ring (Part 2) due to radiation protection regulations.
Schedule: 11.11. - 15.11.2024, Meeting point at 10:00 am at the gatekeeper
Location: c/o Soft Matter and Functional Materials, Elektronenspeicherring BESSY II, Albert-Einstein-Str. 15, 12489 Berlin, Adlershof
Part 3: Daumke
Schedule: 18.11. - 21.11..2024
Location: MDC für Molekulare Medizin, Robert-Rössle-Str. 10. 13125 Berlin (Buch), Seminar: MDC.C (Haus 83). Dendrit 2; Praktikum: Haus 31.2, Raum 0248 (AG Heinemann) (s.Vorlesungsverzeichnis)
close
Comments
Method module: Fundamentals of Structural Biochemistry
University/Department/Institute: Freie Universität Berlin/Department of Biology, Chemistry, Pharmacy/Institute of Chemistry and Biochemistry
Person responsible for the module: Lecturers of the module
Admission requirements: none
Qualification goals: Students learn methods for the preparation of biological macromolecules and for structural analysis. They acquire the theoretical basics of macromolecular structural analysis methods. Students are able to assess macromolecular properties that play a role in structural analysis. They will be able to critically evaluate manuscripts in which macromolecular structures and structural analyses are described and assess the quality of macromolecular structures. Contents: Preparation of a sample of a biological macromolecule for structural analysis; bioinformatic, biochemical or biophysical characterization of a biological macromolecule; performance of one or more procedures for structural analysis; structural description and graphical representation of structures; presentation of structural biology experiments and results.
Content of the seminar:
Protein production and characterization
Protein crystallization and protein crystals
Crystallographic symmetry
X-ray diffraction theory
The phase problem and phasing strategies
Electron density, model building, refinement, validation
close
University/Department/Institute: Freie Universität Berlin/Department of Biology, Chemistry, Pharmacy/Institute of Chemistry and Biochemistry
Person responsible for the module: Lecturers of the module
Admission requirements: none
Qualification goals: Students learn methods for the preparation of biological macromolecules and for structural analysis. They acquire the theoretical basics of macromolecular structural analysis methods. Students are able to assess macromolecular properties that play a role in structural analysis. They will be able to critically evaluate manuscripts in which macromolecular structures and structural analyses are described and assess the quality of macromolecular structures. Contents: Preparation of a sample of a biological macromolecule for structural analysis; bioinformatic, biochemical or biophysical characterization of a biological macromolecule; performance of one or more procedures for structural analysis; structural description and graphical representation of structures; presentation of structural biology experiments and results.
Content of the seminar:
Protein production and characterization
Protein crystallization and protein crystals
Crystallographic symmetry
X-ray diffraction theory
The phase problem and phasing strategies
Electron density, model building, refinement, validation
close
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