216404a Seminar

Seminar on Bioanalytical Mass Spectrometry / Proteomic Analysis

Benno Kuropka

Comments

 

  • Fundamentals of proteome analysis
  • Technical basics of a mass spectrometer
  • Sample preparation and chromatographic peptide separation
  • Ionization methods (ESI and MALDI)
  • Protein identification by MALDI peptide mass fingerprinting
  • Methods for protein analysis (top-down, bottom-up, targeted)
  • Fragmentation methods and interpretation of MS/MS spectra for sequence determination
  • Methods of quantitative proteome analysis including different labeling techniques
  • Identification of protein interactions by affinity-purification MS
  • Analysis of non-covalent protein interactions using native MS
  • Guest Seminars on different topics: e.g. MALDI imaging, crosslinking-MS, carbohydrate analysis, metabolomics
Dr. Benno Kuropka: kuropka@zedat.fu-berlin.de close

Subjects A - Z