216621b Laboratory Course

Analyzing musculoskeletal development in vivo

Sigmar Stricker

Information for students

Recommendation: It is recommended to complete module 216701 a, b "Introduction to developmental biology" before taking the methods module.

Attendance of the seminar is obligatory for the attendance of the practical course

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Comments

Content:
  • Dissection of laboratory mice
  • Tissue embedding, cryoconservation
  • Cryosectioning of tissue
  • Histological methods; Hematoxylin/Eosin staining, Oil Red O staining of tissue sections
  • Alizarin red / Alcian blue staining of skeletal preparations
  • PCR-Genotyping of genetically modified laboratory mice
  • Immunohistochemistry
  • mRNA in-situ hybridization
  • imaging of whole-embryo specimen
  • imaging of histological sections
  • imaging of fluorescently labelled tissue sections


Prof. Dr. S. Stricker: sigmar.stricker@fu-berlin.de close

Subjects A - Z